Pyrococcus furiosus Argonaute-mediated nucleic acid detection

Ruyi He; Longyu Wang; Fei Wang; Wenqiang Li; Yang Liu; Aitao Li; Yuan Wang; Wuxiang Mao; Chao Zhai; Lixin Ma

doi:10.1039/C9CC07339F

Pyrococcus furiosus Argonaute-mediated nucleic acid detection†

Ruyi He,

^a Longyu Wang,^a Fei Wang,^a Wenqiang Li,^a Yang Liu,^a Aitao Li,^a Yuan Wang,^b Wuxiang Mao,^a Chao Zhai*^a and Lixin Ma*^a

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* Corresponding authors

^a State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei Key Laboratory of Industrial Biotechnology, School of Life Sciences, Hubei University, Wuhan, Hubei, People's Republic of China
E-mail: malixing@hubu.edu.cn, chaozhai@hubu.edu.cn

^b Medical College of Hubei University of Arts and Sciences, Xiangyang, Hubei, People's Republic of China

Abstract

We demonstrate that short single-stranded DNA generated by Pyrococcus furiosus Argonaute (PfAgo) can initiate a second round of cleavage. Based on this principle, we established a molecular diagnostic method, termed PfAgo-mediated Nucleic acid Detection (PAND). This method could detect DNA at attomolar sensitivities, distinguish single-nucleotide mutants and accomplish multiplexed detection.

Chemical Communications

Pyrococcus furiosus Argonaute-mediated nucleic acid detection†

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