Issue 45, 2019

SERS-based particle tracking and molecular imaging in live cells: toward the monitoring of intracellular dynamics

Abstract

Although diverse endogenous biomolecules involved in life processes are of major interest in cell biology, there is still a lack of suitable methods for studying biomolecules within live cells without labelling. Herein, we describe a near-infrared (NIR) surface-enhanced Raman scattering (SERS)-based particle tracking technique gathering chemical information inside live cells for monitoring their intracellular dynamics. The wide-field SERS imaging spectroscopy system facilitates high temporal resolution (200 ms) under high spatial resolution (512 × 512 pixels) for one live cell. With high spatiotemporal resolution and signal-to-background ratio, we show that the Raman signal from intracellular cargoes in live cells is sporadically observed and localized to a vesicular level.

Graphical abstract: SERS-based particle tracking and molecular imaging in live cells: toward the monitoring of intracellular dynamics

Supplementary files

Article information

Article type
Communication
Submitted
18 Jun 2019
Accepted
10 Aug 2019
First published
07 Sep 2019

Nanoscale, 2019,11, 21724-21727

SERS-based particle tracking and molecular imaging in live cells: toward the monitoring of intracellular dynamics

J. Kim, S. H. Nam, D. Lim and Y. D. Suh, Nanoscale, 2019, 11, 21724 DOI: 10.1039/C9NR05159G

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