Atomic force microscopy-based single-molecule force spectroscopy detects DNA base mismatches

Wenjing Liu; Yourong Guo; Kaizhe Wang; Xingfei Zhou; Ying Wang; Junhong Lü; Zhifeng Shao; Jun Hu; Daniel M. Czajkowsky; Bin Li

doi:10.1039/C9NR05234H

Atomic force microscopy-based single-molecule force spectroscopy detects DNA base mismatches†

Wenjing Liu,

‡^ab Yourong Guo,‡^c Kaizhe Wang,

‡^ab Xingfei Zhou,^d Ying Wang,^ae Junhong Lü,^ae Zhifeng Shao,^c Jun Hu,

*^aef Daniel M. Czajkowsky*^c and Bin Li

*^ae

Author affiliations

* Corresponding authors

^a Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China
E-mail: hujun@sinap.ac.cn, libin@sinap.ac.cn

^b University of Chinese Academy of Sciences, Beijing 100049, China

^c Bio-ID Center, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China
E-mail: dczaj@sjtu.edu.cn

^d School of Science, Ningbo University, Ningbo 315211, Zhejiang, China

^e Zhangjiang Laboratory, Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201210, China

^f School of Physical Science and Technology, Shanghai Tech University, Shanghai 201204, China

Abstract

Atomic force microscopy-based single-molecule-force spectroscopy is limited by low throughput. We introduce addressable DNA origami to study multiple target molecules. Six target DNAs that differed by only a single base-pair mismatch were clearly differentiated a rupture force of only 4 pN.

Nanoscale

Atomic force microscopy-based single-molecule force spectroscopy detects DNA base mismatches†

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