Efficient synthesis and characterisation of the amyloid beta peptide, Aβ1–42, using a double linker system

Johanes K. Kasim; Iman Kavianinia; Jin Ng; Paul W. R. Harris; Nigel P. Birch; Margaret A. Brimble

doi:10.1039/C8OB02929F

Efficient synthesis and characterisation of the amyloid beta peptide, Aβ_1–42, using a double linker system†

Johanes K. Kasim,

^a Iman Kavianinia,

^abc Jin Ng,^ad Paul W. R. Harris,

*^abc Nigel P. Birch^ad and Margaret A. Brimble

*^abc

Author affiliations

* Corresponding authors

^a School of Biological Sciences, The University of Auckland, 3A Symonds Street, Auckland 1010, New Zealand
E-mail: paul.harris@auckland.ac.nz

^b Maurice Wilkins Centre for Molecular Biodiscovery, The University of Auckland, 3 Symonds St, Auckland 1010, New Zealand

^c School of Chemical Sciences, The University of Auckland, 23 Symonds Street, Auckland 1010, New Zealand
E-mail: m.brimble@auckland.ac.nz

^d Brain Research New Zealand Rangahau Roro Aotearoa and Centre for Brain Research, Auckland 1010, New Zealand

Abstract

The amyloidogenic Aβ₄₂ peptide was efficiently prepared using a double linker system, markedly improving solubility and chromatographic peak resolution, thus enabling full characterisation using standard techniques. The tag was readily cleaved with sodium hydroxide and removed by aqueous extraction, affording Aβ₄₂ in high purity and yield for biophysical characterisation studies.

This article is part of the themed collection: Chemical Biology in OBC

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Article information

DOI: https://doi.org/10.1039/C8OB02929F
Article type: Communication
Submitted: 23 Nov 2018
Accepted: 26 Nov 2018
First published: 26 Nov 2018
This article is Open Access

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Org. Biomol. Chem., 2019,17, 30-34

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Efficient synthesis and characterisation of the amyloid beta peptide, Aβ_1–42, using a double linker system

J. K. Kasim, I. Kavianinia, J. Ng, P. W. R. Harris, N. P. Birch and M. A. Brimble, Org. Biomol. Chem., 2019, 17, 30 DOI: 10.1039/C8OB02929F

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Organic & Biomolecular Chemistry