Issue 25, 2019

Rational design of a function-based selection method for genetically encoding acylated lysine derivatives

Abstract

A foundation of genetic code expansion is the evolution of orthogonal aminoacyl-tRNA synthetase to recognize a non-canonical amino acid, which typically involves read-through of amber stop codon in the genes used in selection. The process is time-consuming, labor-intensive, and susceptible to certain bias. As a complementation, herein, we rationally designed a function-based method to directed evolution of aminoacyl-tRNA synthetase for acylated lysine derivatives.

Graphical abstract: Rational design of a function-based selection method for genetically encoding acylated lysine derivatives

Supplementary files

Article information

Article type
Communication
Submitted
30 Apr 2019
Accepted
04 Jun 2019
First published
05 Jun 2019

Org. Biomol. Chem., 2019,17, 6127-6130

Rational design of a function-based selection method for genetically encoding acylated lysine derivatives

H. Miao, C. Yu, A. Yao and W. Xuan, Org. Biomol. Chem., 2019, 17, 6127 DOI: 10.1039/C9OB00992B

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