An NIR-triggered drug release and highly efficient photodynamic therapy from PCL/PNIPAm/porphyrin modified graphene oxide nanoparticles with the Janus morphology

Abstract

This project aimed to investigate the synthesis and characteristics of stimuli-responsive nanoparticles with different morphologies. In the first step, graphene oxide was synthesized based on the improved Hummers' method. Then, thermo-responsive poly(N-isopropylacrylamide-co-N-(hydroxymethyl)acrylamide), an amphiphilic copolymer, and poly(caprolactone) (PCL), a hydrophobic polymer, were used to prepare Janus and mixed graphene oxide-based nanoparticles. Fluorescence microscopy was utilized to confirm the Janus structure by labeling the mixed and Janus NPs with fluorescent hydrophobic and hydrophilic dyes via a solvent-evaporation method. Then, terminally modified carboxyl porphyrin (TPPC3-COOH), used as the second generation photosensitizer, was grafted to the copolymer surrounding the mixed and Janus NPs. Next, quercetin, a hydrophobic anti-cancer drug, was loaded onto both NPs to accomplish NIR-triggered photodynamic- and chemo-therapy. Finally, the drug loading, encapsulation efficiency, and in vitro release of thermo-responsive NPs were investigated at temperatures of 37 °C and 40 °C as well as under laser irradiation (808 nm).