Issue 3, 2019

Two-photon fluorescence imaging reveals a Golgi apparatus superoxide anion-mediated hepatic ischaemia-reperfusion signalling pathway

Abstract

Hepatic ischaemia-reperfusion (IR) injury is mainly attributed to a burst of reactive oxygen species (ROS) that attack biological macromolecules and lead to cell death. The superoxide anion (O2˙) is the first ROS to be generated and triggers the production of other ROS; thus, explorations of the role of O2˙ in the IR process are meaningful. Meanwhile, the Golgi apparatus generates O2˙via Golgi-associated proteins, which might play an essential role in IR injury. However, the molecular mechanism by which O2˙ from the Golgi apparatus regulates hepatic IR injury is unclear. Therefore, to solve this problem, a two-photon (TP) excited fluorescence probe (CCA) was designed and prepared for the reversible detection of O2˙ in the Golgi apparatus. With the assistance of TP fluorescence microscopy, we observed a substantial increase in the levels of O2˙ in the Golgi apparatus of an IR mouse liver for the first time, as well as increased caspase-2 activity and apoptosis. Furthermore, we found that the tumour necrosis factor (TNF-α) functions as a positive mediator of O2˙ generation. Based on these data, we identified the potential signalling pathway in the Golgi that mediates O2˙ fluctuations in IR mice and revealed the related molecular mechanisms; we also provide a new target for treating IR injury.

Graphical abstract: Two-photon fluorescence imaging reveals a Golgi apparatus superoxide anion-mediated hepatic ischaemia-reperfusion signalling pathway

Supplementary files

Article information

Article type
Edge Article
Submitted
03 Sep 2018
Accepted
01 Nov 2018
First published
06 Nov 2018
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry
Creative Commons BY-NC license

Chem. Sci., 2019,10, 879-883

Two-photon fluorescence imaging reveals a Golgi apparatus superoxide anion-mediated hepatic ischaemia-reperfusion signalling pathway

W. Zhang, J. Zhang, P. Li, J. Liu, D. Su and B. Tang, Chem. Sci., 2019, 10, 879 DOI: 10.1039/C8SC03917H

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