In situ sulfur-doped graphitic carbon nitride nanosheets with enhanced electrogenerated chemiluminescence used for sensitive and selective sensing of l-cysteine

Abstract

In this study, in situ sulfur-doped carbon nitride nanosheets (S-g-C₃N₄ NSs) are used as the signal readout for the sensitive and selective sensing of L-cysteine (L-Cys) in human serum samples based on the competitive coordination chemistry of Cu²⁺ between L-Cys and S-g-C₃N₄ NSs. S-g-C₃N₄ NSs are prepared by using trithiocyanuric acid as a precursor for the first time, which exhibits stronger electrogenerated chemiluminescence (ECL) intensity compared with pristine graphitic carbon nitride nanosheets (g-C₃N₄ NSs). The ECL signals of the S-g-C₃N₄ NSs can be quenched by Cu²⁺ and the subsequent presence of L-Cys can recover the ECL signals of the S-g-C₃N₄ NSs. These changes are ascribed to the higher coordination ability between Cu²⁺ and L-Cys than that between Cu²⁺ and the S-g-C₃N₄ NSs. On the basis of this, the concentration of L-Cys can be quantitatively determined. Under optimized conditions, the ECL intensity recovery shows a linear relationship with the L-Cys concentration range from 30 nM to 0.2 mM with a lower detection limit of 5 nM (S/N = 3). The proposed method is highly sensitive and selective and is thus particularly useful for fast and simple clinical diagnosis of diseases, such as Alzheimer's disease and cardiovascular disease.