Issue 34, 2019

One-step sensitive thrombin detection based on a nanofibrous sensing platform

Abstract

Convenient and time-saving one-step strategies for detecting ultralow concentrations of protein biomarkers play key roles in rapid disease diagnosis. In this study, we report a one-step detection method based on a nanofibrous sensing platform via the combination of proximity-induced DNA strand displacement (PiDSD), catalytic hairpin assembly (CHA) amplification and thioflavin T (ThT) binding. The interface behaviors on the nanofibrous membrane were studied to promote interface reaction kinetics and thermodynamics. Thrombin was used as a model biomarker, and the nanofibrous sensing platform achieved a limit of detection as low as 1.0 pM, a wide linear range of 50 pM to 5 nM, excellent specificity and good long-term stability. Compared with previous one-step thrombin detection methods, our one-step detection method is label-free, convenient and much more sensitive; it has potential applications for protein detection in point-to-care testing (POCT) and early diagnosis.

Graphical abstract: One-step sensitive thrombin detection based on a nanofibrous sensing platform

Supplementary files

Article information

Article type
Paper
Submitted
31 May 2019
Accepted
20 Jul 2019
First published
22 Jul 2019

J. Mater. Chem. B, 2019,7, 5161-5169

One-step sensitive thrombin detection based on a nanofibrous sensing platform

X. Li, Y. Wu, J. Niu, D. Jiang, D. Xiao and C. Zhou, J. Mater. Chem. B, 2019, 7, 5161 DOI: 10.1039/C9TB01098J

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