Determination of membrane capacitance and cytoplasm conductivity by simultaneous electrorotation

Abstract

Membrane capacitances and cytoplasm conductivities of hematopoietic cells were investigated by simultaneous electrorotation (ROT) systems of multiple cells. Simultaneous ROT was achieved by the rotation of electric fields in grid arrays formed with three-dimensional interdigitated array (3D-IDA) electrodes that can be easily fabricated using two substrates with IDA electrodes. When AC signals were applied to four microband electrodes with a 90° phase difference to each electrode, cells dispersed randomly in the 3D-IDA device started to rotate and moved to the center of each grid. Multiple cells were simultaneously rotated at the center of grids without friction from contact with other cells and substrates. The averages and variance of ROT rates of cells at each frequency can be measured during a single operation of the device within 5 min, resulting in the acquisition of ROT spectra. Membrane capacitances and cytoplasm conductivities of hematopoietic cells (K562 cells, Jurkat cells, and THP-1 cells) were determined by fitting ROT spectra obtained experimentally to the curves calculated theoretically. The values determined by using the simultaneous ROT systems well coincided with the values reported previously. The membrane capacitances and cytoplasm conductivities of WEHI-231 cells were firstly determined to be 8.89 ± 0.25 mF m⁻² and 0.28 ± 0.03 S m⁻¹, respectively. Furthermore, the difference of the ROT rates based on the difference of the electric properties of cells was applied to discriminate the types of cells. The acquisition of rotation rates of multiple cells within a single operation makes the statistical analysis extremely profitable for determining the electrical properties of cells.