A ratiometric fluorescent probe for imaging enzyme dependent hydrogen sulfide variation in the mitochondria and in living mice

Abstract

Developing a ratiometric H₂S fluorescent probe with fast response is of great importance for studying the H₂S physiology. Herein, two hemicyanine-based H₂S probes were constructed; the one with a propanoic acid group (CouPa) showed poor sensitivity while the other one with the N,N-diethylpropionamide moiety (CouDE) exhibited distinctly improved performance. CouDE showed the ability to detect mitochondrial H₂S level fluctuation, which was triggered by alteration of CBS enzyme activity. Moreover, endogenous H₂S change in solid tumours was monitored using CouDE.