Issue 23, 2020
From the journal:

Analyst

A cytosolically localized far-red to near-infrared rhodamine-based fluorescent probe for calcium ions†

Koji Numasawa,a   Kenjiro Hanaoka, ORCID logo *a   Takayuki Ikeno,a   Honami Echizen,a   Tomoe Ishikawa,a   Masakazu Morimoto, ORCID logo b   Toru Komatsu, ORCID logo a   Tasuku Ueno, ORCID logo a   Yuji Ikegaya,a   Tetsuo Naganoc  and  Yasuteru Urano*ade  

* Corresponding authors

a Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan
E-mail: uranokun@mol.f.u-tokyo.ac.jp, khanaoka@mol.f.u-tokyo.ac.jp

b Department of Chemistry and Research Center for Smart Molecules, Rikkyo University, 3-34-1 Nishi-Ikebukuro, Toshima-ku, Tokyo 171-8501, Japan

c Drug Discovery Initiative, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan

d Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan

e CREST (Japan) Agency for Medical Research and Development (AMED), 1-7-1 Otemachi, Chiyoda-ku, Tokyo 100-0004, Japan

Abstract

Ca2+ is one of the most important second messengers in cells. A far-red to near-infrared (NIR) Ca2+ fluorescent probe is useful for multi-color imaging in GFP or YFP-expressing biosamples. Here we developed a cytosolically localized far-red to NIR rhodamine-based fluorescent probe for Ca2+, CaSiR-2 AM, while rhodamine dyes are basically localized to mitochondria or lysosomes in cells.

Graphical abstract: A cytosolically localized far-red to near-infrared rhodamine-based fluorescent probe for calcium ions

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Article information

DOI
https://doi.org/10.1039/D0AN01739F
Article type
Paper
Submitted
31 Aug 2020
Accepted
22 Sep 2020
First published
23 Sep 2020

Analyst, 2020,145, 7736-7740

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A cytosolically localized far-red to near-infrared rhodamine-based fluorescent probe for calcium ions

K. Numasawa, K. Hanaoka, T. Ikeno, H. Echizen, T. Ishikawa, M. Morimoto, T. Komatsu, T. Ueno, Y. Ikegaya, T. Nagano and Y. Urano, Analyst, 2020, 145, 7736 DOI: 10.1039/D0AN01739F

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