Issue 4, 2020
From the journal:

Chemical Communications

DNAzyme-powered nucleic acid release from solid supports†

Ting Cao, ORCID logo abc   Yongcheng Wang,abd   Ye Tao,a   Lexiang Zhang,a   Ying-Lin Zhou, ORCID logo *c   Xin-Xiang Zhang,*c   John A. Heyman*a  and  David A. Weitz ORCID logo *ab  

* Corresponding authors

a John A. Paulson School of Engineering and Applied Sciences and Department of Physics, Harvard University, Cambridge, MA 02138, USA
E-mail: jheyman@g.harvard.edu, weitz@seas.harvard.edu

b Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA 02115, USA

c Beijing National Laboratory for Molecular Sciences (BNLMS), MOE Key Laboratory of Bioorganic Chemistry and Molecular Engineering, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China
E-mail: zhouyl@pku.edu.cn, zxx@pku.edu.cn

d Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA

Abstract

Here, we demonstrate use of a Mg2+-dependent, site-specific DNA enzyme (DNAzyme) to cleave oligos from polyacrylamide gel beads, which is suitable for use in drop-based assays. We show that cleavage efficiency is improved by use of a tandem-repeat cleavage site. We further demonstrate that DNAzyme-released oligos function as primers in reverse transcription of cell-released mRNA.

Graphical abstract: DNAzyme-powered nucleic acid release from solid supports

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Article information

DOI
https://doi.org/10.1039/C9CC07790A
Article type
Communication
Submitted
04 Oct 2019
Accepted
26 Nov 2019
First published
13 Dec 2019

Chem. Commun., 2020,56, 647-650

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DNAzyme-powered nucleic acid release from solid supports

T. Cao, Y. Wang, Y. Tao, L. Zhang, Y. Zhou, X. Zhang, J. A. Heyman and D. A. Weitz, Chem. Commun., 2020, 56, 647 DOI: 10.1039/C9CC07790A

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