A fluorescent nanobiosensor for the facile analysis of m6A RNA demethylase activity

Seo-Hee Yim; Hyung Jin Cha; Se-Jin Park; Yeajee Yim; Jae-Sung Woo; Dal-Hee Min

doi:10.1039/C9CC10054G

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A fluorescent nanobiosensor for the facile analysis of m⁶A RNA demethylase activity†

Seo-Hee Yim,‡^a Hyung Jin Cha,

‡^bc Se-Jin Park,^a Yeajee Yim,^a Jae-Sung Woo^bc and Dal-Hee Min

*^ade

Author affiliations

* Corresponding authors

^a Department of Chemistry, Seoul National University, Seoul 08826, Republic of Korea
E-mail: dalheemin@snu.ac.kr

^b Centre for RNA Research, Institute for Basic Science (IBS), Seoul National University, Seoul 08826, Republic of Korea

^c Department of Life Sciences, Korea University, Seoul 02841, Republic of Korea

^d Department of Biological Sciences, Seoul National University, Seoul 08826, Republic of Korea

^e Institute of Biotherapeutics Convergence Technology, Lemonex Inc., Seoul 08826, Republic of Korea

Abstract

RNA demethylase has recently been known to be associated with cancer development but its selective inhibitors as anti-cancer agents have rarely been investigated to date. Herein, we have developed a fluorescent nanobiosensor which enables efficient quantitative analysis of RNA demethylase ALKBH5 activity and shows a high potential for robust inhibitor screening.

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Article information

DOI: https://doi.org/10.1039/C9CC10054G
Article type: Communication
Submitted: 30 Dec 2019
Accepted: 17 Mar 2020
First published: 18 Mar 2020

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Chem. Commun., 2020,56, 4716-4719

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A fluorescent nanobiosensor for the facile analysis of m⁶A RNA demethylase activity

S. Yim, H. J. Cha, S. Park, Y. Yim, J. Woo and D. Min, Chem. Commun., 2020, 56, 4716 DOI: 10.1039/C9CC10054G

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