High-affinity and undissociated capillary electrophoresis for DNA strand exchange analysis

Fangzhi Yu; Zheng Yuan; Dapeng Zhang; Yan Liu; Qiang Zhao; Hailin Wang

doi:10.1039/D0CC02844D

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High-affinity and undissociated capillary electrophoresis for DNA strand exchange analysis†

Fangzhi Yu,

^ab Zheng Yuan,

^ab Dapeng Zhang,

^ab Yan Liu,

^ab Qiang Zhao

^ab and Hailin Wang

*^abc

Author affiliations

* Corresponding authors

^a State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, No. 18 Shuangqing Road, Beijing 100085, P. R. China
E-mail: hlwang@rcees.ac.cn

^b University of Chinese Academy of Sciences, No. 19A Yuquan Road, Beijing 100049, P. R. China

^c Institute of Environment and Health, Jianghan University, No. 8, Sanjiaohu Road, Wuhan, Hubei, P. R. China

Abstract

By identification of a super-stable protein–DNA-affinity system, we developed a free-solution capillary electrophoresis approach for rapid and sensitive detection of fundamentally important DNA strand exchange reactions mediated by recombinases. We further extended this assay for identification of hyper-recombinases generated from bioengineering and detection of single DNA mismatches caused by replication error.

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Article information

DOI: https://doi.org/10.1039/D0CC02844D
Article type: Communication
Submitted: 20 Apr 2020
Accepted: 01 Jun 2020
First published: 01 Jun 2020

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Chem. Commun., 2020,56, 7403-7406

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High-affinity and undissociated capillary electrophoresis for DNA strand exchange analysis

F. Yu, Z. Yuan, D. Zhang, Y. Liu, Q. Zhao and H. Wang, Chem. Commun., 2020, 56, 7403 DOI: 10.1039/D0CC02844D

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Chemical Communications

High-affinity and undissociated capillary electrophoresis for DNA strand exchange analysis†

Abstract

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High-affinity and undissociated capillary electrophoresis for DNA strand exchange analysis

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