A double-stranded DNA catalyzed strand displacement system for detection of small genetic variations

Na Liu; Xuzhe Zhang; Xiaofeng Tang; Yizhou Liu; Donghui Huang; Xianjin Xiao

doi:10.1039/D0CC06216B

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A double-stranded DNA catalyzed strand displacement system for detection of small genetic variations†

Na Liu,

‡^a Xuzhe Zhang,

‡^a Xiaofeng Tang,

^a Yizhou Liu,

^a Donghui Huang

*^a and Xianjin Xiao

*^a

Author affiliations

* Corresponding authors

^a Institute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, P. R. China
E-mail: xiaoxianjin@hust.edu.cn

Abstract

A double-stranded DNA catalyzed strand displacement system (dsCSD) was established for the detection of small genetic variations, which showed greatly enhanced specificity compared to the conventional single-stranded DNA catalyzed strand displacement (ssCSD) system. The system achieved limits of detection (LODs) of 0.05% and 0.1% for synthesized DNA samples and clinical gene samples, respectively.

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Article information

DOI: https://doi.org/10.1039/D0CC06216B
Article type: Communication
Submitted: 17 Sep 2020
Accepted: 21 Oct 2020
First published: 22 Oct 2020

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Chem. Commun., 2020,56, 14397-14400

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A double-stranded DNA catalyzed strand displacement system for detection of small genetic variations

N. Liu, X. Zhang, X. Tang, Y. Liu, D. Huang and X. Xiao, Chem. Commun., 2020, 56, 14397 DOI: 10.1039/D0CC06216B

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Chemical Communications

A double-stranded DNA catalyzed strand displacement system for detection of small genetic variations†

Abstract

Supplementary files

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A double-stranded DNA catalyzed strand displacement system for detection of small genetic variations

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