Issue 4, 2020

The purification, structural characterization and antidiabetic activity of a polysaccharide from Anoectochilus roxburghii

Abstract

Anoectochilus roxburghii, a traditional Chinese medicinal herb, has been widely used for treating numerous chronic diseases. In this study, a polysaccharide from A. roxburghii (ARPs-p) was purified by anion exchange and size exclusion chromatography. The structural characteristics of ARPs-p were systematically investigated for the first time via numerous chromatographic techniques, periodic acid oxidation, Smith degradation, methylation analysis, FTIR spectroscopy and 1D/2D NMR spectroscopy. The results showed that ARPs-p is a heteropolysaccharide with a molecular weight of 97 kDa; it consists of 97.75% glucose, 1.2% galactose and trace amounts of galacturonic acid, and its backbone structure is composed of →3)-β-D-Glcp-(1→ with some branching points at O-6 linked to non-reducing end units or 6-O-linked Glcp units. Furthermore, streptozotocin (STZ)-induced diabetic mouse experiments suggested that ARPs-p has excellent antihyperglycemic, antioxidant and antihyperlipidemic activities, in which 1,3-β-D-glucan, the main component of ARPs-p, plays a vital role.

Graphical abstract: The purification, structural characterization and antidiabetic activity of a polysaccharide from Anoectochilus roxburghii

Article information

Article type
Paper
Submitted
24 Apr 2019
Accepted
06 Apr 2020
First published
21 Apr 2020

Food Funct., 2020,11, 3730-3740

The purification, structural characterization and antidiabetic activity of a polysaccharide from Anoectochilus roxburghii

Y. Liu, T. Tang, S. Duan, C. Li, Q. Lin, H. Wu, A. Liu, B. Hu, D. Wu, S. Li, L. Shen and W. Wu, Food Funct., 2020, 11, 3730 DOI: 10.1039/C9FO00860H

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