Issue 20, 2020
From the journal:

Green Chemistry

Light-driven deracemization of phosphinothricin by engineered fatty acid photodecarboxylase on a gram scale†

Feng Cheng, ORCID logo ab   Heng Li, ORCID logo ab   Dong-Yang Wu,ab   Ju-Mou Li, ORCID logo ab   Yi Fan,ab   Ya-Ping Xue ORCID logo *ab  and  Yu-Guo Zheng ORCID logo ab  

* Corresponding authors

a The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, Hangzhou, P. R. China
E-mail: xyp@zjut.edu.cn

b , Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310014, P. R. China

Abstract

A fatty acid photodecarboxylase (CvFAP) based photobiocatalytic deracemization of phosphinothricin (PPT) was developed. The engineered CvFAP enabled the kinetic resolution of racemic PPT on a gram scale, providing unreacted L-PPT with high yields and excellent stereoselectivity. In contrast to previous biocatalytic approaches, this efficient light-driven process required neither NADPH recycling nor a PPT precursor.

Graphical abstract: Light-driven deracemization of phosphinothricin by engineered fatty acid photodecarboxylase on a gram scale

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Article information

DOI
https://doi.org/10.1039/D0GC02696D
Article type
Communication
Submitted
05 Aug 2020
Accepted
24 Sep 2020
First published
29 Sep 2020

Green Chem., 2020,22, 6815-6818

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Light-driven deracemization of phosphinothricin by engineered fatty acid photodecarboxylase on a gram scale

F. Cheng, H. Li, D. Wu, J. Li, Y. Fan, Y. Xue and Y. Zheng, Green Chem., 2020, 22, 6815 DOI: 10.1039/D0GC02696D

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