Development and characterisation of acoustofluidic devices using detachable electrodes made from PCB

Roman Mikhaylov; Fangda Wu; Hanlin Wang; Aled Clayton; Chao Sun; Zhihua Xie; Dongfang Liang; Yinhua Dong; Fan Yuan; Despina Moschou; Zhenlin Wu; Ming Hong Shen; Jian Yang; Yongqing Fu; Zhiyong Yang; Christian Burton; Rachel J. Errington; Marie Wiltshire; Xin Yang

doi:10.1039/C9LC01192G

Development and characterisation of acoustofluidic devices using detachable electrodes made from PCB†

Roman Mikhaylov,^a Fangda Wu,^a Hanlin Wang,^a Aled Clayton,^b Chao Sun,^c Zhihua Xie,^d Dongfang Liang,^e Yinhua Dong,^f Fan Yuan,^g Despina Moschou,^h Zhenlin Wu,ⁱ Ming Hong Shen,^j Jian Yang,^j Yongqing Fu,

^k Zhiyong Yang,^l Christian Burton,^ab Rachel J. Errington,^b Marie Wiltshire^b and Xin Yang

^a

Author affiliations

^a Department of Electrical and Electronic Engineering, School of Engineering, Cardiff University, Cardiff CF24 3AA, UK

^b Tissue Micro-Environment Group, Division of Cancer & Genetics, School of Medicine, Cardiff University, Cardiff CF14 4XN, UK

^c School of Life Sciences, Northwestern Polytechnical University, P.R. China

^d Department of Civil Engineering, School of Engineering, Cardiff University, Cardiff CF24 3AA, UK

^e Department of Engineering, University of Cambridge, Cambridge CB2 1PZ, UK

^f Department of Neurology, Tianjin 4th Centre Hospital Affiliated to Nankai University, P.R. China

^g Department of Biomedical Engineering, School of Engineering, Duke University, NC 27708-0281, USA

^h Centre for Biosensors, Bioelectronics and Biodevices (C3Bio) and Department of Electronic & Electrical Engineering, University of Bath, Bath BA2 7AY, UK

ⁱ School of Optoelectronic Engineering and Instrumentation Science, Dalian University of Technology, P.R. China

^j Preclinical Studies of Renal Tumours Group, Division of Cancer and Genetics, School of Medicine, Cardiff University, Cardiff CF14 4XN, UK

^k Faculty of Engineering and Environment, Northumbria University, Newcastle Upon Tyne, Newcastle NE1 8ST, UK

^l School of Mechanical Engineering, Tianjin University, P.R. China

Abstract

Acoustofluidics has been increasingly applied in biology, medicine and chemistry due to its versatility in manipulating fluids, cells and nano-/micro-particles. In this paper, we develop a novel and simple technology to fabricate a surface acoustic wave (SAW)-based acoustofluidic device by clamping electrodes made using a printed circuit board (PCB) with a piezoelectric substrate. The PCB-based SAW (PCB-SAW) device is systematically characterised and benchmarked with a SAW device made using the conventional photolithography process with the same specifications. Microparticle manipulations such as streaming in droplets and patterning in microchannels were demonstrated in the PCB-SAW device. In addition, the PCB-SAW device was applied as an acoustic tweezer to pattern lung cancer cells to form three or four traces inside the microchannel in a controllable manner. Cell viability of ∼97% was achieved after acoustic manipulation using the PCB-SAW device, which proved its ability as a suitable tool for acoustophoretic applications.

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Development and characterisation of acoustofluidic devices using detachable electrodes made from PCB†

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