Synergistic integration of FeNi magnetic nanoparticles with graphene-based porous carbon for efficient capture of N-linked glycans

Abstract

Developing functional porous carbon is greatly desired for the capture of N-glycans from complex bio-samples. In this work, ferronickel graphene-based porous carbon composites (FeNi–G/PC-T, T = carbonization temperature) are facilely prepared and are characterized by the synergistic integration of magnetic separation, porosity and polar interaction. Studies of capture of N-linked glycans reveal that FeNi–G/PC-800 shows a remarkable performance to enrich N-linked glycans from standard bio-samples and real human serum, resulting in the successful profiling of 48 N-linked glycans in 5 μL human serum. Structure–property relationship studies further demonstrate that the synergistically integrated FeNi nanoparticles and graphene-based porous carbon in FeNi–G/PC-800 should play a key role in the capture performance.