Issue 28, 2020
From the journal:

Organic & Biomolecular Chemistry

Genetic dereplication driven discovery of a tricinoloniol acid biosynthetic pathway in Trichoderma hypoxylon†

Huan Liu, ORCID logo ab   Yu-Han Pu,c   Jin-Wei Ren,b   Er-Wei Li,b   Li-Xia Guoc  and  Wen-Bing Yin ORCID logo *ab  

* Corresponding authors

a School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230027, PR China
E-mail: yinwb@im.ac.cn

b State Key Laboratory of Mycology and CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China

c Environmental and Resources Institute, Chongqing Technology and Business University, Chongqing 400067, PR China

Abstract

A genetic dereplication approach in combination with differential gene expression led to the discovery of three new sesquiterpenes, tricinoloniol acids (TRAs) A–C (1–3) and the known fusidilactone A (4) from T. hypoxylon. Comparative transcriptomic analysis and targeted deletion identified the biosynthetic route for TRAs. Our results demonstrate an alternative application of the genetic dereplication method for exploring the biosynthesis of cryptic secondary metabolites (SMs), which utilizes the coordinated expression of trichothecene (tri) and tra cluster genes.

Graphical abstract: Genetic dereplication driven discovery of a tricinoloniol acid biosynthetic pathway in Trichoderma hypoxylon

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Article information

DOI
https://doi.org/10.1039/D0OB01202E
Article type
Communication
Submitted
10 Jun 2020
Accepted
27 Jun 2020
First published
29 Jun 2020

Org. Biomol. Chem., 2020,18, 5344-5348

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Genetic dereplication driven discovery of a tricinoloniol acid biosynthetic pathway in Trichoderma hypoxylon

H. Liu, Y. Pu, J. Ren, E. Li, L. Guo and W. Yin, Org. Biomol. Chem., 2020, 18, 5344 DOI: 10.1039/D0OB01202E

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