Issue 40, 2020

l-DNA-tagged fluorescence in situ hybridization for highly sensitive imaging of RNAs in single cells

Abstract

We report an effective fluorescence in situ hybridization strategy, named L-DNA tagged FISH (LT-FISH), for highly sensitive RNA detection in fixed cultured cells. LT-FISH includes two-step hybridization processes with a LD chimera oligonucleotide probe and a fluorescence-labeled PCR product tethering a L-DNA tag. The degree of fluorescence enhancement, depending on the length of PCR products, was up to 14-fold when the 606 bp product was used. Endogenous mRNA and miRNA in cancer cells were visualized by utilizing this L-DNA-mediated signal amplification technique.

Graphical abstract: l-DNA-tagged fluorescence in situ hybridization for highly sensitive imaging of RNAs in single cells

Supplementary files

Article information

Article type
Communication
Submitted
07 Aug 2020
Accepted
22 Sep 2020
First published
22 Sep 2020

Org. Biomol. Chem., 2020,18, 8084-8088

Author version available

L-DNA-tagged fluorescence in situ hybridization for highly sensitive imaging of RNAs in single cells

M. Ogata, G. Hayashi, A. Ichiu and A. Okamoto, Org. Biomol. Chem., 2020, 18, 8084 DOI: 10.1039/D0OB01635G

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