Issue 50, 2020
From the journal:

RSC Advances

Parallel G-quadruplex-mediated protein dimerization and activation†

Tuom Tinh Thi Truong,a   Cuong Caob  and  Dung Thanh Dang ORCID logo *ac  

* Corresponding authors

a University of Science, Vietnam National University, Ho Chi Minh City, Vietnam
E-mail: dung.dthanh@ou.edu.vn

b School of Biological Sciences, Queen's University Belfast, UK

c Ho Chi Minh City Open University, Ho Chi Minh City, Vietnam

Abstract

We studied parallel G4-mediated protein dimerization and activation by incorporating a RHAU peptide with a fluorescent protein FRET pair CFP/YFP and an apoptotic casp9. Occurrence of energy tranfer (from donor CFP to acceptor YFP) and enhancement of 60-fold cleavage efficiency of casp9 were observed in the presence of parallel G4, which indicated that parallel G4 can induce dimerization and activation of proteins. This novel approach holds a great promise for studying G4-targeting functional dimeric proteins in celllular biology.

Graphical abstract: Parallel G-quadruplex-mediated protein dimerization and activation

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/D0RA06173E
Article type
Paper
Submitted
15 Jul 2020
Accepted
07 Aug 2020
First published
13 Aug 2020
This article is Open Access
Creative Commons BY-NC license

RSC Adv., 2020,10, 29957-29960

Permissions

Request permissions

Parallel G-quadruplex-mediated protein dimerization and activation

T. T. Thi Truong, C. Cao and D. T. Dang, RSC Adv., 2020, 10, 29957 DOI: 10.1039/D0RA06173E

This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. You can use material from this article in other publications, without requesting further permission from the RSC, provided that the correct acknowledgement is given and it is not used for commercial purposes.

To request permission to reproduce material from this article in a commercial publication, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party commercial publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements