AuNPs@PMo12 nanozyme: highly oxidase mimetic activity for sensitive and specific colorimetric detection of acetaminophen

Abstract

The design of a highly specific and sensitive approach for the quantitative and qualitative determination of acetaminophen (AP) is crucial from a human health point of view. In this study, AuNPs@PMo₁₂, as a nanozyme, has been developed for the highly sensitive and selective detection of AP with 3,3′,5,5′-tetramethylbenzidine (TMB) within a few seconds without adding oxidizing reagents (e.g. H₂O₂). Synthesized nanosensors are able to oxidize TMB to yellow-brown oxidized TMB (oxTMB). The maximum peak wavelength of oxTMB was observed at 450 nm. The addition of AP and then increasing its concentration led to the production of different products in blue color. In experimental measurements, the limit of detection was obtained as 14.52 mg L⁻¹. The quantitative determination of AP concentrations can be carried out using UV-vis spectroscopy. The design of nanosensors is cost-effective and application of them in H₂O₂-free and enzyme-free conditions provides a rapid sensing approach for practical use in disease monitoring and diagnosis.