Fluorescent molecularly imprinted nanoparticles with boronate affinity for selective glycoprotein detection

Abstract

Specific recognition and sensing of glycoproteins are of great importance in clinical diagnostics considering their frequent utilization as biomarkers and therapeutic targets. In this work, a biomimetic fluorescent sensor for the selective and sensitive detection of glycoprotein was developed, which was based on late-model boronate fluorescent molecularly imprinted nanoparticles (B-FMIP NPs). The B-FMIP NPs were fabricated via the macromolecular assembly of a fluorescent photo-crosslinkable amphiphilic copolymer containing boronic acid with glycoprotein in aqueous solution and in situ photo-crosslinking. Due to the synergism of boronate affinity and the molecular imprinting effect, the resultant B-FMIP NPs demonstrated specific recognition and remarkable selectivity toward the template glycoprotein (ovalbumin, OVA) with a high imprinted factor (α) of 6.0 and gave rise to obvious fluorescence quenching after binding with OVA in water. Under optimized experimental conditions, the as-prepared B-FMIP NPs exhibited linearity over the OVA concentration range of 10⁻¹³ to 10⁻³ mg mL⁻¹ with a detection limit of 3.3 × 10⁻¹⁴ mg mL⁻¹, as well as a rapid response time (about 10 min), which was superior to that of other previously reported OVA sensors. Finally, these B-FMIP NPs have been applied for the determination of OVA in real samples.