Production of a monoclonal antibody for the detection of vitamin B1 and its use in an indirect enzyme-linked immunosorbent assay and immunochromatographic strip

Abstract

A monoclonal antibody (mAb) against vitamin B₁ was prepared and based on this, an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and colloidal gold-based immunochromatographic test (ICT) strip were developed. The 50% inhibition concentration (IC₅₀) and the limit of detection (LOD) for the developed ic-ELISA were estimated to be 20.71 ng mL⁻¹ and 4.51 ng mL⁻¹ in the assay buffer. Vitamin B₁ in energy drinks, vitamin tablets, and infant milk powder samples was detected by the developed ic-ELISA and the average recoveries ranged from 80.63% to 103.05%. The vitamin B₁ content in energy drinks, vitamin tablets, and infant milk powder samples were confirmed by using liquid chromatography tandem mass spectrometry (LC-MS/MS). Moreover, the cut-off value of the developed ICT strip assay was 250 ng mL⁻¹, with a LOD value of 5.33 ng mL⁻¹. Real samples were used to validate the reliability of the developed ICT strip. All results demonstrated that our developed methods are suitable for the detection of vitamin B₁ in food and pharmaceutical products.