Issue 44, 2020

Tailoring cellular microenvironments using scaffolds based on magnetically-responsive polymer brushes

Abstract

A variety of polymeric scaffolds with the ability to control cell detachment has been created for cell culture using stimuli-responsive polymers. However, the widely studied and commonly used thermo-responsive polymeric substrates always affect the properties of the cultured cells due to the temperature stimulus. Here, we present a different stimuli-responsive approach based on poly(3-acrylamidopropyl)trimethylammonium chloride) (poly(APTAC)) brushes with homogeneously embedded superparamagnetic iron oxide nanoparticles (SPIONs). Neuroblastoma cell detachment was triggered by an external magnetic field, enabling a non-invasive process of controlled transfer into a new place without additional mechanical scratching and chemical/biochemical compound treatment. Hybrid scaffolds obtained in simultaneous surface-initiated atom transfer radical polymerization (SI-ATRP) were characterized by atomic force microscopy (AFM) working in the magnetic mode, secondary ion mass spectrometry (SIMS), and X-ray photoelectron spectroscopy (XPS) to confirm the magnetic properties and chemical structure. Moreover, neuroblastoma cells were cultured and characterized before and after exposure to a neodymium magnet. Controlled cell transfer triggered by a magnetic field is presented here as well.

Graphical abstract: Tailoring cellular microenvironments using scaffolds based on magnetically-responsive polymer brushes

Supplementary files

Article information

Article type
Paper
Submitted
30 Jul 2020
Accepted
11 Oct 2020
First published
21 Oct 2020
This article is Open Access
Creative Commons BY-NC license

J. Mater. Chem. B, 2020,8, 10172-10181

Tailoring cellular microenvironments using scaffolds based on magnetically-responsive polymer brushes

W. Górka-Kumik, P. Garbacz, D. Lachowicz, P. Dąbczyński, S. Zapotoczny and M. Szuwarzyński, J. Mater. Chem. B, 2020, 8, 10172 DOI: 10.1039/D0TB01853H

This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. You can use material from this article in other publications, without requesting further permission from the RSC, provided that the correct acknowledgement is given and it is not used for commercial purposes.

To request permission to reproduce material from this article in a commercial publication, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party commercial publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements