Installing CRISPR–Cas12a sensors in a portable glucose meter for point-of-care detection of analytes

Ran Liu; Ying He; Tian Lan; Jingjing Zhang

doi:10.1039/D1AN00008J

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Installing CRISPR–Cas12a sensors in a portable glucose meter for point-of-care detection of analytes†

Ran Liu,

‡^a Ying He,‡^a Tian Lan^b and Jingjing Zhang

*^a

Author affiliations

* Corresponding authors

^a State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Chemistry and Biomedicine Innovation Center (ChemBIC), Nanjing University, Nanjing 210023, China
E-mail: jing15209791@nju.edu.cn

^b GlucoSentient, Inc. 2100 S. Oak Street, Suite 101, Champaign, IL 61820, USA

Abstract

Integrating CRISPR–Cas12a sensors with a portable glucose meter (PGM) was developed based on the target-induced activation of the collateral cleavage activity of Cas12a. Considering the portability, low cost and facile incorporation of the PGM system with suitable Cas12a sensors to recognize many targets, the CRISPR/Cas12a-PGM system demonstrated here paves a way to further broaden the POC applications of CRISPR-based diagnostics.

This article is part of the themed collections: Analyst Recent HOT articles and Celebrating 100 years of Chemistry at Nanjing University

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Article information

DOI: https://doi.org/10.1039/D1AN00008J
Article type: Communication
Submitted: 03 Jan 2021
Accepted: 24 Mar 2021
First published: 25 Mar 2021

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Analyst, 2021,146, 3114-3120

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Installing CRISPR–Cas12a sensors in a portable glucose meter for point-of-care detection of analytes

R. Liu, Y. He, T. Lan and J. Zhang, Analyst, 2021, 146, 3114 DOI: 10.1039/D1AN00008J

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Analyst

Installing CRISPR–Cas12a sensors in a portable glucose meter for point-of-care detection of analytes†

Abstract

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Installing CRISPR–Cas12a sensors in a portable glucose meter for point-of-care detection of analytes

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