Issue 13, 2021

Determination of seven tetracyclines in milk by dissolvable layered double hydroxide-based solid-phase extraction coupled with high-performance liquid chromatography

Abstract

A dissolvable layered double hydroxide-based solid-phase extraction combined with high-performance liquid chromatography was developed for the analysis of minocycline, oxytetracycline, tetracycline, demeclocycline, metacycline, chlortetracycline and doxycycline in milk samples. In situ formation of the layered double hydroxide was achieved by the addition of MgCl2–AlCl3 solution to alkaline deproteinized milk. The analytes were efficiently extracted by the Mg/Al layered double hydroxide. After centrifugation, the co-precipitates were dissolved in 0.1 mol L−1 Na2EDTA–McIlvaine buffer prior to HPLC analysis. Under optimized conditions, the method achieved low detection limits of 0.414–0.986 μg L−1 and quantification limits of 1.38–3.29 μg L−1, and good recoveries of 93.5–100% with intra- and inter-day RSDs of 0.498–4.08% and 1.23–10.0%, respectively. This method is convenient, accurate, sensitive, rapid, cost-effective, eco-friendly, and suitable for the determination of seven tetracycline antibiotics in milk samples.

Graphical abstract: Determination of seven tetracyclines in milk by dissolvable layered double hydroxide-based solid-phase extraction coupled with high-performance liquid chromatography

Supplementary files

Article information

Article type
Paper
Submitted
28 Jan 2021
Accepted
25 Feb 2021
First published
26 Feb 2021

Anal. Methods, 2021,13, 1618-1624

Determination of seven tetracyclines in milk by dissolvable layered double hydroxide-based solid-phase extraction coupled with high-performance liquid chromatography

Y. Yang, J. Zhang, Y. Li, S. Yin, Y. Jiang and C. Sun, Anal. Methods, 2021, 13, 1618 DOI: 10.1039/D1AY00154J

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements