Issue 11, 2021

An acoustic field-based conformal transfection system for improving the gene delivery efficiency

Abstract

Ultrasound-activated microbubble destruction is a promising platform for gene delivery due to the low toxicity, non-invasiveness, and high specificity. However, the gene transfection efficiency is still low, especially for suspension cells. It is desirable to develop a universal gene delivery tool that overcomes the drawbacks existing in ultrasound-mediated methods. Here, we present a three-dimensional acoustic field-based conformal transfection (AFCT) system by designing a Sono-hole that can fit the three-dimensional acoustic field to maximally utilize the acoustic energy from bubble cavitation, thus greatly promoting the gene delivery efficiency. Surprisingly, compared with the traditional two-dimensional transfection system, the gene transfection efficiency of the AFCT system increased by more than 3 times, achieving nearly 30%. The parameters including acoustic pressure, duration, duty cycle, DNA concentrations, and bubble kinds were optimized to obtain higher gene transfection. In conclusion, our study provides an effective ultrasound-based gene delivery approach for gene transfection, especially for suspension-cultured cells.

Graphical abstract: An acoustic field-based conformal transfection system for improving the gene delivery efficiency

Article information

Article type
Paper
Submitted
11 Feb 2021
Accepted
06 Apr 2021
First published
14 Apr 2021

Biomater. Sci., 2021,9, 4127-4138

An acoustic field-based conformal transfection system for improving the gene delivery efficiency

L. Xie, J. Wang, S. Zhao, M. L. Lai, T. Jiang and F. Yan, Biomater. Sci., 2021, 9, 4127 DOI: 10.1039/D1BM00251A

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