Issue 45, 2021
From the journal:

Chemical Communications

Construction of an optically controllable CRISPR-Cas9 system using a DNA origami nanostructure†

Katsuhiko Abe,a   Hiroshi Sugiyama ORCID logo *ab  and  Masayuki Endo ORCID logo *abc  

* Corresponding authors

a Department of Chemistry, Graduate School of Science, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto 606-8502, Japan
E-mail: hs@kuchem.kyoto-u.ac.jp

b Institute for Integrated Cell-Material Sciences, Kyoto University, Yoshida-ushinomiyacho, Sakyo-ku, Kyoto 606-8501, Japan

c Organization for Research and Development of Innovative Science and Technology, Kansai University, Suita, Osaka 564-8680, Japan
E-mail: endo@kansai-u.ac.jp

Abstract

We demonstrated the photo-controlled sequence-selective dsDNA cleavage using a DNA origami structure with Cas9 nuclease. The activity of Cas9 incorporated inside the ring-shaped DNA origami was completely suppressed. After photoirradiation, Cas9 was released, and its activity for selective dsDNA cleavage was restored.

Graphical abstract: Construction of an optically controllable CRISPR-Cas9 system using a DNA origami nanostructure

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Article information

DOI
https://doi.org/10.1039/D1CC00876E
Article type
Communication
Submitted
16 Feb 2021
Accepted
30 Apr 2021
First published
07 May 2021

Chem. Commun., 2021,57, 5594-5596

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Construction of an optically controllable CRISPR-Cas9 system using a DNA origami nanostructure

K. Abe, H. Sugiyama and M. Endo, Chem. Commun., 2021, 57, 5594 DOI: 10.1039/D1CC00876E

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