Direct ligand screening against membrane proteins on live cells enabled by DNA-programmed affinity labelling

Yiran Huang; Yuqing Deng; Jianfu Zhang; Ling Meng; Xiaoyu Li

doi:10.1039/D1CC00961C

You do not have JavaScript enabled. Please enable JavaScript to access the full features of the site or access our non-JavaScript page.

Direct ligand screening against membrane proteins on live cells enabled by DNA-programmed affinity labelling†

Yiran Huang,^a Yuqing Deng,^a Jianfu Zhang,^a Ling Meng^a and Xiaoyu Li

*^a

Author affiliations

* Corresponding authors

^a Department of Chemistry and State Key Laboratory of Synthetic Chemistry, The University of Hong Kong, Pokfulam Road, Hong Kong SAR, China, Laboratory for Synthetic Chemistry and Chemical Biology, Health@InnoHK, Innovation and Technology Commission, Hong Kong, SAR, China
E-mail: xiaoyuli@hku.hk

Abstract

Membrane proteins are important drug targets; however, ligand discovery for membrane proteins is highly challenging due to their hydrophobic nature. We show that membrane proteins may be specifically labelled with a DNA tag by DNA-programmed affinity labelling (DPAL), thereby enabling the screening of chemical compounds against membrane proteins directly on live cells.

Download options Please wait...

Supplementary files

Article information

DOI: https://doi.org/10.1039/D1CC00961C
Article type: Communication
Submitted: 22 Feb 2021
Accepted: 11 Mar 2021
First published: 12 Mar 2021

Download Citation

Chem. Commun., 2021,57, 3769-3772

Permissions

Request permissions

Direct ligand screening against membrane proteins on live cells enabled by DNA-programmed affinity labelling

Y. Huang, Y. Deng, J. Zhang, L. Meng and X. Li, Chem. Commun., 2021, 57, 3769 DOI: 10.1039/D1CC00961C

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Social activity

Fetching data from CrossRef.
This may take some time to load.

Chemical Communications

Direct ligand screening against membrane proteins on live cells enabled by DNA-programmed affinity labelling†

Abstract

Supplementary files

Article information

Download Citation

Permissions

Direct ligand screening against membrane proteins on live cells enabled by DNA-programmed affinity labelling

Social activity

Search articles by author

Spotlight

Advertisements