A sequentially activated bioluminescent probe for observation of cellular H2O2 production induced by cysteine

Kaiqing Ma; Yongkang Yue; Lingling Zhao; Jianbin Chao; Caixia Yin

doi:10.1039/D1CC04015D

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A sequentially activated bioluminescent probe for observation of cellular H₂O₂ production induced by cysteine†

Kaiqing Ma,

‡^a Yongkang Yue,‡^a Lingling Zhao,^a Jianbin Chao

^b and Caixia Yin

*^a

Author affiliations

* Corresponding authors

^a Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Key Laboratory of Materials for Energy Conversion and Storage of Shanxi Province, Institute of Molecular Science, Shanxi University, Taiyuan 030006, China
E-mail: yincx@sxu.edu.cn

^b Research Institute of Applied Chemistry, Shanxi University, Taiyuan 030006, China

Abstract

We report herein a caged luciferin probe Cy-Hy as a sequentially activated probe to selectively and sensitively sense L-Cys and H₂O₂. The probe displayed fluorescence and bioluminescence responses toward the two analytes. Utilizing the present probe, cellular excess L-Cys-induced H₂O₂ up-regulation was observed for the first time in living MDA-MB-231 cells.

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Article information

DOI: https://doi.org/10.1039/D1CC04015D
Article type: Communication
Submitted: 24 Jul 2021
Accepted: 03 Sep 2021
First published: 03 Sep 2021

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Chem. Commun., 2021,57, 10015-10018

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A sequentially activated bioluminescent probe for observation of cellular H₂O₂ production induced by cysteine

K. Ma, Y. Yue, L. Zhao, J. Chao and C. Yin, Chem. Commun., 2021, 57, 10015 DOI: 10.1039/D1CC04015D

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Chemical Communications