Keratin-inorganic hybrid nanoflowers decorated with Fe3O4 nanoparticles as enzyme mimics for colorimetric detection of glucose

Abstract

Fe₃O₄ magnetic nanoparticles (MNPs) are highly active enzyme-like catalysts. However, low stability is still a big challenge for Fe₃O₄-based enzyme mimics because the Fe₃O₄ MNPs can be easily dissolved when exposed to acidic conditions. Inspired by the numerous catalytic sites of a flower-like structure and the biological functions of amino acids in structural proteins, herein, by employing keratin as a protein component, stable Fe₃O₄-based MNP embedded keratin-Cu₃(PO₄)₂ nanoflowers were constructed, from which hierarchical nanostructures with a three-dimensional petal-like morphology were selected for subsequent studies owing to their excellent enzymic catalytic activity. The keratin–nanoflower@Fe₃O₄ exhibited significantly enhanced catalytic activity compared with that of keratin-Cu₃(PO₄)₂ nanoflowers and individual Fe₃O₄ MNPs. Remarkably, keratin-nanoflower@Fe₃O₄ exhibited superior long-term stability to Fe₃O₄ MNPs under more acidic conditions and favorable reusability. This method has been successfully exploited for the colorimetric determination of glucose in human serum with satisfactory sensitivity and specificity, offering a novel approach for glucose detection.