Physicochemical properties and digestive kinetics of whey protein gels filled with potato and whey protein mixture emulsified oil droplets: effect of protein ratios

Abstract

Incorporating protein emulsified droplets into protein gels as active fillers have attracted much attention. However, using animal and plant protein mixtures emulsified droplets as the filler is lacking. We investigated the effect of emulsified droplets covered by potato protein (PP) and whey protein (WP) mixtures of different ratios (10/0, 9/1, 7/3, 5/5, 3/7, 1/9, 0/10) on mechanical, microstructural characteristics and digestion of emulsion-filled WP gels (EFWG). The results showed that the particle size of emulsified droplets increased with the enhancement of PP ratio, whereas their ζ-potential value decreased. Increasing the PP ratio improved the elastic moduli (G′), fracture stress and hardness of EFWG, while lowered the water holding capacity and swelling ratios of EFWG. Confocal laser scanning microscopy revealed that a higher PP ratio leads to a thicker gel skeleton and fine network. Although the enhancement of the PP ratio decreased disulfide bond content in EFWG, it improved the hydrogen bond and total non-covalent interactions in EFWG. Increased PP in filling emulsions delayed the release rate of the free amino group and free fatty acid during digestion. Moreover, the presence of NaCl improved the gel properties and digestion of EFWG. The findings of this study may provide information for developing new WP gel products with specific digestion rates.