Pectic polysaccharide from Nelumbo nucifera leaves promotes intestinal antioxidant defense in vitro and in vivo

Chao Huang; Xi Peng; De-Jiang Pang; Juan Li; Berit Smestad Paulsen; Frode Rise; Yu-Long Chen; Zheng-Li Chen; Ren-Yong Jia; Li-Xia Li; Xu Song; Bin Feng; Zhong-Qiong Yin; Yuan-Feng Zou

doi:10.1039/D1FO02354C

Pectic polysaccharide from Nelumbo nucifera leaves promotes intestinal antioxidant defense in vitro and in vivo†

Chao Huang,‡^abc Xi Peng,‡^a De-Jiang Pang,‡^d Juan Li,^e Berit Smestad Paulsen,^f Frode Rise,^g Yu-Long Chen,*^h Zheng-Li Chen,^bc Ren-Yong Jia,^c Li-Xia Li,^a Xu Song,^a Bin Feng,

ⁱ Zhong-Qiong Yin

^a and Yuan-Feng Zou

*^bc

Author affiliations

* Corresponding authors

^a Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R. China

^b Laboratory of Experimental Animal Disease Model, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R. China
E-mail: yuanfengzou@sicau.edu.cn
Fax: +86-28-8629-1470
Tel: +86-28-8629-1470

^c Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R. China

^d Neuroscience & Metabolism Research, Sichuan University and Collaborative Innovation Center, Chengdu 610041, China

^e Institute of Animal Science; Chengdu Academy of Agriculture and Forestry Sciences, Chengdu, Sichuan Province, China

^f Department of Pharmacy, Section Pharmaceutical Chemistry, Area Pharmacognosy, University of Oslo, P.O. Box 1068, Blindern, 0316 Oslo, Norway

^g Department of Chemistry, University of Oslo, P.O. Box 1033, Blindern, 0315 Oslo, Norway

^h Sichuan Academy of Forestry, Ecological Restoration and Conservation on Forest and Wetland Key Laboratory of Sichuan Province. Chengdu, Sichuan, China
E-mail: ww9987@163.com
Fax: +86-28-83429472
Tel: +86-28-83429472

ⁱ Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, P.R. China

Abstract

In this study, the Nelumbo nucifera leaf polysaccharide (NNLP) was isolated by hot water extraction and ethanol precipitation. DEAE anion exchange chromatography and gel filtration were further performed to obtained the purified fraction NNLP-I-I, the molecular weight of which was 16.4 kDa. The monosaccharide composition analysis and linkage units determination showed that the fraction NNLP-I-I was a pectic polysaccharide. In addition, the NMR spectra analysis revealed that NNLP-I-I mainly consisted of a homogalacturonan backbone and rhamnogalacturonan I, containing a long HG region and short RG-I region, with AG-II and 1–3 linked rhamnose as side chains. The biological studies demonstrated that NNLP-I-I displayed antioxidant properties through mediating the Nrf2-regulated intestinal cellular antioxidant defense, which could protect cultured intestinal cells from oxidative stress and improve the intestinal function of aged mice.

Food & Function

Pectic polysaccharide from Nelumbo nucifera leaves promotes intestinal antioxidant defense in vitro and in vivo†

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Pectic polysaccharide from Nelumbo nucifera leaves promotes intestinal antioxidant defense in vitro and in vivo

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