Colorimetric detection of hypoxanthine in aquatic products based on the enzyme mimic of cobalt-doped carbon nitride

Abstract

Rapid detection of hypoxanthine (Hx), an important freshness indicator of aquatic products, is still urgent and challenging. In the present study, a colorimetric method for the rapid detection of Hx in aquatic products was established based on the peroxidase-like activity of cobalt-doped graphite phase carbon nitride (Co-doped-g-C₃N₄). After calcination of the mixture of melamine, cobalt chloride, potassium chloride and sodium chloride, Co-doped-g-C₃N₄ was obtained with strong peroxidase-like activity and good dispersity. Through the decomposition of Hx by xanthine oxidase, the generated hydrogen peroxide (H₂O₂) was detected based on the oxidation reaction of H₂O₂ and 3,3′,5,5′-tetramethylbenzidine catalyzed by Co-doped-g-C₃N₄. The content of Hx could be directly measured by monitoring the spectral absorbance at 652 nm with a linear range of 2.50–153.1 mg kg⁻¹ and a detection limit of 1.84 mg kg⁻¹ calculated based on 3σ/K (n = 9). The established method was applied for the detection of Hx content in various aquatic products at different storage times after execution, and the data were validated by the HPLC method. The results showed that the Hx measurement by the established method was sensitive, accurate and reliable, and could be used for the rapid determination of the freshness of aquatic products at an early stage.