Issue 31, 2021
From the journal:

Organic & Biomolecular Chemistry

Inhibition of off-target cleavage by RNase H using an artificial cationic oligosaccharide†

Rintaro Iwata Hara ORCID logo *ab  and  Takeshi Wada ORCID logo *a  

* Corresponding authors

a Department of Medicinal and Life Sciences, Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan
E-mail: twada@rs.tus.ac.jp

b Department of Neurology and Neurological Science, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45, Yushima, Bunkyo-Ku, Tokyo, Japan
E-mail: haranuro@tmd.ac.jp

Abstract

Sequence-dependent off-target effects are a serious problem of antisense oligonucleotide-based drugs. Some of these side effects are induced by ribonuclease H (RNase H)-mediated cleavage of non-target RNAs with base sequences similar to that of the target RNA. We found that an artificial cationic oligosaccharide, ODAGal4, improved single-base discrimination for RNase H cleavage.

Graphical abstract: Inhibition of off-target cleavage by RNase H using an artificial cationic oligosaccharide

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Article information

DOI
https://doi.org/10.1039/D1OB00983D
Article type
Paper
Submitted
21 May 2021
Accepted
15 Jul 2021
First published
23 Jul 2021
This article is Open Access
Creative Commons BY-NC license

Org. Biomol. Chem., 2021,19, 6865-6870

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Inhibition of off-target cleavage by RNase H using an artificial cationic oligosaccharide

R. I. Hara and T. Wada, Org. Biomol. Chem., 2021, 19, 6865 DOI: 10.1039/D1OB00983D

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