Issue 38, 2021
From the journal:

Organic & Biomolecular Chemistry

Deuteration of terminal alkynes realizes simultaneous live cell Raman imaging of similar alkyne-tagged biomolecules†

Syusuke Egoshi, ORCID logo a   Kosuke Dodo, ORCID logo *ab   Kenji Ohgane ORCID logo a  and  Mikiko Sodeoka ORCID logo *ab  

* Corresponding authors

a Synthetic Organic Chemistry Laboratory, RIKEN Cluster for Pioneering Research, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan
E-mail: dodo@riken.jp, sodeoka@riken.jp

b RIKEN Center for Sustainable Resource Science, 2-1, Hirosawa, Wako, Saitama 351-0198, Japan

Abstract

Alkynes were employed as tags to observe small molecules in cells by Raman microscopy. Herein, simple deuteration was found to shift the vibrational frequency of the alkyne by 135 cm−1. Two-color Raman imaging of D-alkynes and H-alkynes made it possible to distinguish between and observe similar small molecules in live cells.

Graphical abstract: Deuteration of terminal alkynes realizes simultaneous live cell Raman imaging of similar alkyne-tagged biomolecules

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Article information

DOI
https://doi.org/10.1039/D1OB01479J
Article type
Communication
Submitted
28 Jul 2021
Accepted
26 Aug 2021
First published
16 Sep 2021
This article is Open Access
Creative Commons BY-NC license

Org. Biomol. Chem., 2021,19, 8232-8236

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Deuteration of terminal alkynes realizes simultaneous live cell Raman imaging of similar alkyne-tagged biomolecules

S. Egoshi, K. Dodo, K. Ohgane and M. Sodeoka, Org. Biomol. Chem., 2021, 19, 8232 DOI: 10.1039/D1OB01479J

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