One-pot analysis of enantiomeric excess of free amino acids by electrospray ionization mass spectrometry

Takashi Nakakoji; Hirofumi Sato; Daisuke Ono; Hiroyuki Miyake; Eiko Mieda; Satoshi Shinoda; Hiroshi Tsukube; Hideya Kawasaki; Ryuichi Arakawa; Motohiro Shizuma

doi:10.1039/D1RA06542D

One-pot analysis of enantiomeric excess of free amino acids by electrospray ionization mass spectrometry†

Takashi Nakakoji,^a Hirofumi Sato,^b Daisuke Ono,^b Hiroyuki Miyake,*^a Eiko Mieda,^a Satoshi Shinoda,

^a Hiroshi Tsukube,^a Hideya Kawasaki,

^c Ryuichi Arakawa^c and Motohiro Shizuma

*^b

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* Corresponding authors

^a Department of Chemistry, Graduate School of Science, Osaka City University, Sugimoto, Sumiyoshi-ku, Osaka 558-8585, Japan
E-mail: miyake@sci.osaka-cu.ac.jp

^b Osaka Research Institute of Industrial Science and Technology, Morinomiya, Joto-ku, Osaka 536-8553, Japan
E-mail: shizuma@omtri.or.jp

^c Department of Chemistry and Materials Engineering, Faculty of Chemistry, Materials and Bioengineering, Kansai University, Yamate-cho, Suita, Osaka 564-8680, Japan

Abstract

An electrospray ionization mass spectrometric method for the simultaneous analysis of the enantiomeric excess of free amino acids, without chromatographic separation, was demonstrated using a quasi-racemic mixture of deuterium-labelled and unlabelled chiral copper(II) complexes. This convenient method enables the simultaneous high-sensitivity determination of the enantiomeric excess of 12 amino acids.

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One-pot analysis of enantiomeric excess of free amino acids by electrospray ionization mass spectrometry†

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One-pot analysis of enantiomeric excess of free amino acids by electrospray ionization mass spectrometry

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