Issue 5, 2021

A helical amplification system composed of artificial nucleic acids

Abstract

Herein we report an amplification system of helical excess triggered by nucleic acid hybridization for the first time. It is usually impossible to prepare achiral nanostructures composed of nucleic acids because of their intrinsic chirality. We used serinol nucleic acid (SNA) oligomers for the preparation of achiral nanowires because SNA oligomers with symmetrical sequences are achiral. Nanowire formation was confirmed by atomic force microscopy and size exclusion chromatography. When a chiral nucleic acid with a sequence complementary to SNA was added to the nanostructure, helicity was induced and a strong circular dichroism signal was observed. The SNA nanowire could amplify the helicity of chiral nucleic acids through nucleobase stacks. The SNA nanostructures have potential for use as platforms to detect chiral biomolecules under aqueous conditions because SNA can be readily functionalized and is water-soluble.

Graphical abstract: A helical amplification system composed of artificial nucleic acids

Supplementary files

Article information

Article type
Edge Article
Submitted
23 Sep 2020
Accepted
22 Dec 2020
First published
12 Jan 2021
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry
Creative Commons BY-NC license

Chem. Sci., 2021,12, 1656-1660

A helical amplification system composed of artificial nucleic acids

H. Kashida, K. Nishikawa, W. Shi, T. Miyagawa, H. Yamashita, M. Abe and H. Asanuma, Chem. Sci., 2021, 12, 1656 DOI: 10.1039/D0SC05245K

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