Issue 25, 2021

Blue-conversion of organic dyes produces artifacts in multicolor fluorescence imaging

Abstract

Multicolor fluorescence imaging is a powerful tool visualizing the spatiotemporal relationship among biomolecules. Here, we report that commonly employed organic dyes exhibit a blue-conversion phenomenon, which can produce severe multicolor image artifacts leading to false-positive colocalization by invading predefined spectral windows, as demonstrated in the case study using EGFR and Tensin2. These multicolor image artifacts become much critical in localization-based superresolution microscopy as the blue-converted dyes are photoactivatable. We provide a practical guideline for the use of organic dyes for multicolor imaging to prevent artifacts derived by blue-conversion.

Graphical abstract: Blue-conversion of organic dyes produces artifacts in multicolor fluorescence imaging

Supplementary files

Article information

Article type
Edge Article
Submitted
01 Feb 2021
Accepted
16 May 2021
First published
18 May 2021
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry
Creative Commons BY license

Chem. Sci., 2021,12, 8660-8667

Blue-conversion of organic dyes produces artifacts in multicolor fluorescence imaging

D. Kim, Y. Chang, S. Park, M. G. Jeong, Y. Kwon, K. Zhou, J. Noh, Y. Choi, T. M. Hong, Y. Chang and S. H. Ryu, Chem. Sci., 2021, 12, 8660 DOI: 10.1039/D1SC00612F

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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