Integrated electrochemical lateral flow immunoassays (eLFIAs): recent advances

Abstract

The discovery of electroactive nano-biomaterials and the development of flexible electrodes have increased the interest in applications of integrated electrochemical lateral flow immunoassays (eLFIAs), which integrate electrochemical nanotags and flexible electrodes on test strips that can easily detect small biomolecules. Compared with colorimetric, optical, magnetic and other highly sensitive detection methods, the electrochemical detection technique is well developed with high sensitivity, selectivity and repeatability. Moreover, the increasing compatibility of interfaces with miniature potentiometers has allowed electrochemical sensors to become more integrated, automated and intelligent, highlighting their huge potential in future developments. This review discusses the relevant eLFIA research over the past 20 years. The basic principles, electrode assemblies, electrochemical labeling strategies and electrical signal detection methods are summarized and analyzed in detail. Finally, perspectives on the current challenges facing eLFIA and its future outlook are presented.