Issue 12, 2022
From the journal:

Analyst

Determination of intracellular ascorbic acid using tandem mass spectrometry†

Chizumi Abe, ORCID logo ab   Ohki Higuchi,ac   Akira Matsumoto ORCID logo ‡*de  and  Taiki Miyazawa ORCID logo *a  

* Corresponding authors

a New Industry Creation Hatchery Center (NICHe), Tohoku University, 6-6-10 Aramaki Aza-Aoba, Aoba-ku, Sendai 980-8579, Japan
E-mail: taiki.miyazawa.b3@tohoku.ac.jp

b Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School of Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan

c Biodynamic Plant Institute Co. Ltd, Hokudai Business Spring Bldg, Nishi 12-2-101, Kita 21 joh, Kita-ku, Sapporo, Hokkaido 001-0021, Japan

d Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University (TMDU), 2-3-10 Kanda-Surugadai, Chiyoda-ku, Tokyo 101-0062, Japan
E-mail: matsumoto.bsr@tmd.ac.jp

e Kanagawa Institute of Industrial Science and Technology (KISTEC), Kanagawa 243-0435, Japan

Abstract

Ascorbic acid is involved in a variety of biological events. Nevertheless, its detailed intracellular behavior remains unexplored due to a lack of sensitive analytical methods. Here we report a method using HPLC-ESI-MS/MS achieving the lowest detection limit ever reported (1 pg), to provide mechanistic insight into the intracellular ascorbic acid physiology.

Graphical abstract: Determination of intracellular ascorbic acid using tandem mass spectrometry

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Article information

DOI
https://doi.org/10.1039/D1AN02160E
Article type
Communication
Submitted
30 Nov 2021
Accepted
12 May 2022
First published
18 May 2022

Analyst, 2022,147, 2640-2643

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Determination of intracellular ascorbic acid using tandem mass spectrometry

C. Abe, O. Higuchi, A. Matsumoto and T. Miyazawa, Analyst, 2022, 147, 2640 DOI: 10.1039/D1AN02160E

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