Enzymatic glycoengineering-based spin labelling of cell surface sialoglycans to enable their analysis by electron paramagnetic resonance (EPR) spectroscopy

Mohit Jaiswal; Trang T. Tran; Jiatong Guo; Mingwei Zhou; Josefina Garcia Diaz; Gail E. Fanucci; Zhongwu Guo

doi:10.1039/D1AN02226A

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Enzymatic glycoengineering-based spin labelling of cell surface sialoglycans to enable their analysis by electron paramagnetic resonance (EPR) spectroscopy†

Mohit Jaiswal,^a Trang T. Tran,^a Jiatong Guo,^a Mingwei Zhou,^a Josefina Garcia Diaz,^a Gail E. Fanucci

*^a and Zhongwu Guo

*^a

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* Corresponding authors

^a Department of Chemistry, University of Florida, 214 Leigh Hall, Gainesville, FL 32611, USA
E-mail: zguo@chem.ufl.edu, fanucci@chem.ufl.edu

Abstract

A novel method for spin labelling of sialoglycans on the cell surface is described. C9-Azido sialic acid was linked to glycans on live cells via CSTII-catalysed α2,3-sialylation utilizing azido-sialic acid nucleotide as a sialyl donor, which was followed by attachment of a spin label to the azide via click reaction. It enables the study of cell surface sialoglycans by EPR spectroscopy.

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Article information

DOI: https://doi.org/10.1039/D1AN02226A
Article type: Communication
Submitted: 08 Dec 2021
Accepted: 08 Feb 2022
First published: 09 Feb 2022

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Analyst, 2022,147, 784-788

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Enzymatic glycoengineering-based spin labelling of cell surface sialoglycans to enable their analysis by electron paramagnetic resonance (EPR) spectroscopy

M. Jaiswal, T. T. Tran, J. Guo, M. Zhou, J. Garcia Diaz, G. E. Fanucci and Z. Guo, Analyst, 2022, 147, 784 DOI: 10.1039/D1AN02226A

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Enzymatic glycoengineering-based spin labelling of cell surface sialoglycans to enable their analysis by electron paramagnetic resonance (EPR) spectroscopy†

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Enzymatic glycoengineering-based spin labelling of cell surface sialoglycans to enable their analysis by electron paramagnetic resonance (EPR) spectroscopy

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