Issue 5, 2022
From the journal:

Analyst

Enzymatic glycoengineering-based spin labelling of cell surface sialoglycans to enable their analysis by electron paramagnetic resonance (EPR) spectroscopy†

Mohit Jaiswal,a   Trang T. Tran,a   Jiatong Guo,a   Mingwei Zhou,a   Josefina Garcia Diaz,a   Gail E. Fanucci ORCID logo *a  and  Zhongwu Guo ORCID logo *a  

* Corresponding authors

a Department of Chemistry, University of Florida, 214 Leigh Hall, Gainesville, FL 32611, USA
E-mail: zguo@chem.ufl.edu, fanucci@chem.ufl.edu

Abstract

A novel method for spin labelling of sialoglycans on the cell surface is described. C9-Azido sialic acid was linked to glycans on live cells via CSTII-catalysed α2,3-sialylation utilizing azido-sialic acid nucleotide as a sialyl donor, which was followed by attachment of a spin label to the azide via click reaction. It enables the study of cell surface sialoglycans by EPR spectroscopy.

Graphical abstract: Enzymatic glycoengineering-based spin labelling of cell surface sialoglycans to enable their analysis by electron paramagnetic resonance (EPR) spectroscopy

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Article information

DOI
https://doi.org/10.1039/D1AN02226A
Article type
Communication
Submitted
08 Dec 2021
Accepted
08 Feb 2022
First published
09 Feb 2022

Analyst, 2022,147, 784-788

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Enzymatic glycoengineering-based spin labelling of cell surface sialoglycans to enable their analysis by electron paramagnetic resonance (EPR) spectroscopy

M. Jaiswal, T. T. Tran, J. Guo, M. Zhou, J. Garcia Diaz, G. E. Fanucci and Z. Guo, Analyst, 2022, 147, 784 DOI: 10.1039/D1AN02226A

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