Issue 16, 2022

Living algae detection with a PDMS-liquid chlorophyll fluorescence microfluidic chip filter and a smartphone

Abstract

Building an optical filtration function into a microfluidic chip is a promising way of simplifying the optical detection system of a microfluidic device. In this paper, a PDMS microfluidic chip filter that is capable of transmitting chlorophyll fluorescence and blocking interfering light in the visible wavelength range was developed for living algae detection with a smartphone. The chip was fabricated by sealing a layer of crystal violet solution in a PDMS layer on the top of the Sudan II-doped PDMS slab, which has a straight microchannel. Optimum dye concentrations and thicknesses for the crystal violet solution layer and Sudan II-doped PDMS slab were investigated and determined by spectrum measurements. It was found that the cut-on range of this integrated microfluidic chip is extended to about 625 nm and the transmittance in the chlorophyll fluorescence range (650 nm to 710 nm) is as high as 95%, when 25 mg L−1 Sudan II-doped PDMS slab (with a 3 mm thickness) and 2 mg L−1 crystal violet solution (with a 0.3 mm thickness) were used. Living algae detection using this chlorophyll-fluorescence-filtering PDMS microfluidic chip and a smartphone-based imaging platform was achieved, and the results compared favorably with those using a commercial filter.

Graphical abstract: Living algae detection with a PDMS-liquid chlorophyll fluorescence microfluidic chip filter and a smartphone

Article information

Article type
Paper
Submitted
03 Mar 2022
Accepted
22 Jun 2022
First published
23 Jun 2022

Analyst, 2022,147, 3723-3731

Living algae detection with a PDMS-liquid chlorophyll fluorescence microfluidic chip filter and a smartphone

J. Liu, H. Chang, X. Zhang, S. Chen, Y. Song and D. Li, Analyst, 2022, 147, 3723 DOI: 10.1039/D2AN00375A

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