Semi-quantification of the binding constant based on bond breaking in a combined acoustic–gravitational field†
Abstract
In this study, we propose a semi-quantification method based on breaking bonds between microparticles and glass plates in a combined acoustic–gravitational field. The semi-quantified binding constant values for BSA–ibuprofen, BSA–ciprofloxacin, ConA–glycogen, ConA–mannan, and BSA–naproxen calculated using this method were 7.5 × 103, 1.6 × 104, 2.3 × 105, 2.4 × 106 and 9.0 × 107 M−1, respectively, which were in concord with the reported values.