Issue 24, 2022

Multi analyte sensing of amphiphilic tridentate bis(benzimidazolyl)pyridine incorporated in liposomes and potential application in enzyme assay

Abstract

A liposome based nanosensor Lipo-1 for efficient detection of copper, cyanide (CN) and ATP in a pure aqueous medium has been described. Lipo-1 shows a fluorescence ON–OFF response with copper. However, Lipo-1.Cu (Lipo-1 and copper ensemble) was used for the OFF–ON detection of ATP with nM and CN with μM detection levels, lower than the WHO permissible level for safe drinking. Lipo-1 has better and enhanced binding properties over the counter organic amphiphilic compound Bzimpy-LC, which is not soluble in water. The significant changes in the emission spectra in the presence of Cu2+, CN and ATP ions, as variable inputs, are used to construct INHIBIT and OR logic operations in a nano-scale environment. The fluorescent detection of CN ions with Lipo-1.Cu was used to develop an enzyme assay for β-glucosidase using amygdalin as the substrate. β-Glucosidase enzymatic activity was monitored by the emission OFF–ON signal of the probe Lipo-1.Cu by CN detection. This approach could be an efficient method for developing a fluorescence-based β-glucosidase enzyme assay. A switch ON luminescence response, low detection limit, fast response, 100% aqueous solution, biocompatibility, multi-analyte detection, and improved sensitivity and selectivity of Bzimpy-LC in lipid bilayer membranes are the main features of the nanoprobe Lipo-1. These properties give it a clear advantage for analytical applications.

Graphical abstract: Multi analyte sensing of amphiphilic tridentate bis(benzimidazolyl)pyridine incorporated in liposomes and potential application in enzyme assay

Supplementary files

Article information

Article type
Paper
Submitted
21 Mar 2022
Accepted
09 May 2022
First published
10 May 2022

Anal. Methods, 2022,14, 2357-2367

Multi analyte sensing of amphiphilic tridentate bis(benzimidazolyl)pyridine incorporated in liposomes and potential application in enzyme assay

S. Gadiyaram, N. Sharma, I. V. M. V. Enoch and D. A. Jose, Anal. Methods, 2022, 14, 2357 DOI: 10.1039/D2AY00486K

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