Issue 22, 2022

A NIR fluorescent probe for the specific detection of hypochlorite and its application in vitro and in vivo

Abstract

It is of great necessity to exploit a real-time, highly selective and sensitive method for hypochlorite (ClO) detection in both the environment and living systems because of the complex influence of ClO on health. In this paper, based on the intramolecular charge transfer (ICT) effect, a NIR fluorescent probe (probe DAB) was designed for the accurate detection of ClO, which produced a fluorescence response to ClO with high selectivity and rapid response (within 1 min). The probe DAB could determine ClO over the linear range of 0–80 μM with a low detection limit of 1.46 μM. And the sensing mechanism between the probe and ClO was verified using HPLC and MS. To further prove its practicability, the probe was applied for detecting ClO in actual water samples. In addition, owing to its good sensing properties and low cytotoxicity, probe DAB could be expediently applied to visualize ClO in living cells and zebrafish, and it is expected to be a useful tool for investigating the detailed functions and mechanisms of ClO in living systems.

Graphical abstract: A NIR fluorescent probe for the specific detection of hypochlorite and its application in vitro and in vivo

Supplementary files

Article information

Article type
Communication
Submitted
04 Apr 2022
Accepted
15 Apr 2022
First published
23 Apr 2022

Anal. Methods, 2022,14, 2147-2152

A NIR fluorescent probe for the specific detection of hypochlorite and its application in vitro and in vivo

L. Zhen, J. Lan, S. Zhang, L. Liu, R. Zeng, Y. Chen and Y. Ding, Anal. Methods, 2022, 14, 2147 DOI: 10.1039/D2AY00561A

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