To study GPCR conformational dynamics in live cells, here we report an integrated approach combining enzymatic SNAP-tagging with bioorthogonal chemistry for dual fluorescent labeling of GLP-1R. The resulting GLP-1R conformational biosensors permit a FRET-based analysis of the receptor subdomain movement in response to ligand stimulation in live cells.
T. M. Lewandowski, P. An, C. P. Ramil, M. Fang and Q. Lin, RSC Chem. Biol., 2022, 3, 702 DOI: 10.1039/D2CB00107A
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